Below is a list of the characters that appear in the Afterschool Charisma universe
| (Main characters) 3rd Clone Generation | ||||||
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| Shiro Kamiya | Sigmund Freud | Elizabeth Tudor | Napoleon Bonaparte | |||
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| Joan Of Arc | Adolf Hitler | Marie Curie | Florence Nightingale | |||
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| Ikkyu Sojun | Wolfgang Mozart | Grigori Rasputin | Himiko | |||
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| Albert Einstein | ||||||
| 2nd Clone Generation | ||||||
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| Kai | Elizabeth Tudor (2nd Generation) | St. Kleio loyal Kai | Sigmund Freud (2nd Generation) | |||
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| Albert Einstein (2nd Generation) | ||||||
| 1st Clone Generation | ||||||
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| Eito Kamiya | Leonardo Da Vinci | |||||
| 4th Clone Generation | ||||||
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| Pandora (Marie Curie)(4th Generation) | ||||||
| Non-Clones | ||||||
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File:Blank.png | |||
| Naoto Kuroe | Rockswell | Dr. X | Robert Green | |||
| Other | ||||||
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| The Almighty Dolly | ||||||